Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 regulated the expression of metastasis-related factors via the NFκB pathway. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. a Protein levels of MTA1; cells were collected for WB with MTA1 and GAPDH antibodies. The bands of over-expressed MTA1 were from Additional file : Fig. 2A and down-expressed MTA1 were from Additional file : Fig. 2B with arrow. b Cells were lysed and used in qRT-PCR assays to measure the E-cadherin mRNA expression. Statistical analysis was performed using Student’s t test. b , c A498 cells were transfected with pcDNA3.1-Flag and Flag-MTA1. After 36 h, the Flag-MTA1 group was treated with 10 nM PDTC for 12 h. Then cells were harvested for qRT-PCR evaluation to measure the mRNA expression of MMP2 ( b ) and MMP9 ( c ). Statistical analysis was performed using ANOVA. d A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 48 h, cells were subjected to western blotting analysis using antibodies targeting p-p65, p65 and ACTB. The bands of p-p65, p65 and ACTB were from Additional file : Fig. 3. e, f Image J was used to calculate the gray scanned bands of p-p65 ( b ) and p65 (C). * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; NS not significant

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 promotes A498 cell migration. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, cells in the four conditions were subjected to the wound healing assay. Macrographs were taken under ×100 magnification

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, Transfection, Wound Healing Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 enhances the invasion of A498 cells. a A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, the transwell cell invasion assay using A498 cells was performed, and macrographs were taken under ×40 magnification. Scale bar: 50 µm. b Image J was used to count the transmigrated cells and Student’s t test to analyze differences. ** p < 0.01; *** p < 0.001

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Transfection, Invasion Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: MTA1 regulated the migration and invasion of RCC cells via NF-κB. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, and Flag-MTA1 + PDTC (10 nM). a In the wound healing assay, MTA1 improved the migration of RCC cells, while the addition of the inhibitor PDTC blocked the effect of MTA1 on migration. Scale bar: 100×. b In the transwell assay, MTA1 markedly induced invasion of A498 cells, but compared to the MTA1 group, the invasion of MTA1 + PDTC A498 cells was lower. ** p < 0.01; *** p < 0.001; Scale bar: ×40

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Migration, Transfection, Wound Healing Assay, Transwell Assay

Journal: BMC Urology

Article Title: Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

doi: 10.1186/s12894-020-00731-1

Figure Lengend Snippet: The expression of MTA1 is up-regulated in RCCs. a Immunohistochemistry was used to characterize the expression of MTA1 in RCC and adjacent tissues. MTA1 was highly expressed in ccRCCs, compared to very weak staining in adjacent tissue. b Image Pro Plus was used for the statistical analysis of the positive signal (IOD) of MTA1 expression in ccRCCs and the adjacent tissue. The expression of MTA1 was significantly up-regulated in ccRCCs. ** p < 0.01; scale bar: 100 µm and 50 µm. c The expression of MTA1 in RCC cell lines. Normal renal cell line HEK293T and RCC cell lines A498 and 768-O cell lysates were used in western blotting analysis with MTA1 and GAPDH antibodies. The bands of MTA1 were from Additional file : Fig. 1 and gapdh were from Additional file : Fig. 1

Article Snippet: The human kidney cancer cell line A498 was purchased from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot

Journal: Journal of Healthcare Engineering

Article Title: lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

doi: 10.1155/2022/2279072

Figure Lengend Snippet: LncRNA MSC-AS1 knockdown alleviates HG-induced proliferation, fibrosis, and inflammation of HRMC cells. (a) The expression of MSC-AS1 in serum samples from DN patients and normal subjects ( n = 30). (b) The expression of MSC-AS1 in HG- and NG-treated HRMC cells. (c) The expression of MSC-AS1 in HG-treated HRMC cells with its siRNA. (d, e) Cell proliferation and apoptosis were detected in the NG, HG, and HG + si-MSC-AS1 groups. (f) Cell fibrosis was assessed by the levels of FN, Col IV, and Col I in the NG, HG, and HG + si-MSC-AS1 groups. (g) The release of TNF- α , IL-6, and IL-1 β was examined in the NG, HG, and HG + si-MSC-AS1 groups. ∗ P < 0.05 ∗∗ P < 0.01.

Article Snippet: Human renal mesangial cell line HRMC (Procell, Wuhan, China) was cultured in a matched dedicated complete medium (CM-H067; Procell) at 37°C with 5% CO 2 .

Techniques: Knockdown, Expressing

Journal: Journal of Healthcare Engineering

Article Title: lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

doi: 10.1155/2022/2279072

Figure Lengend Snippet: lncRNA MSC-AS1 functions as a miR-325 sponge. (a) The binding sites between MSC-AS1 and miR-325. (b) The relationship between MSC-AS1 and miR-325 was verified by pull-down assay. (c) MiR-325 expression was detected in HG-treated HRMC cells with MSC-AS1 siRNA and vector. (d) MSC-AS1 expression was measured in HG-treated HRMC cells containing miR-325 mimics or inhibitors. (e) MiR-325 expression was detected in DN patients and normal subjects ( n = 30). (f) A negative correlation between MSC-AS1 and miR-325 expression was found in DN patients ( n = 30). ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Human renal mesangial cell line HRMC (Procell, Wuhan, China) was cultured in a matched dedicated complete medium (CM-H067; Procell) at 37°C with 5% CO 2 .

Techniques: Binding Assay, Pull Down Assay, Expressing, Plasmid Preparation

Journal: Journal of Healthcare Engineering

Article Title: lncRNA MSC-AS1 Aggravates Diabetic Nephropathy by Regulating the miR-325/CCNG1 Axis

doi: 10.1155/2022/2279072

Figure Lengend Snippet: CCNG1 is a direct target of miR-325. (a) The binding sites between miR-325 and CCNG1. (b) The relationship between miR-325 and CCNG1 was confirmed by pull-down assay. (c) CCNG1 expression was measured in HG-treated HRMC cells containing miR-325 mimics or inhibitors. (d) CCNG1 expression was detected in DN patients and normal subjects. (e) A negative correlation between CCNG1 and miR-325 expression was identified in DN patients ( n = 30). (f) A positive correlation between MSC-AS1 and CCNG1 expression was detected in DN patients ( n = 30). (g) CCNG1 expression was assessed in HG-treated HRMC cells with MSC-AS1 siRNA and vector. ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Human renal mesangial cell line HRMC (Procell, Wuhan, China) was cultured in a matched dedicated complete medium (CM-H067; Procell) at 37°C with 5% CO 2 .

Techniques: Binding Assay, Pull Down Assay, Expressing, Plasmid Preparation